Figure 4
Expression profiles of PPRs in the spleen and brain of DPV-infected ducks.
The samples of viral infected ducks were collected at 1, 3 and 5 dpi. Total RNA was extracted and cDNA was prepared for detecting the cytokines. The expressions of cytokines tested in this study were measured using 2−ΔΔCt method by relative quantification. Data were expressed as mean fold change (n = 5). (A) The expression of major PRRs contained the TLR2, TLR3, TLR21, RIG-I and MDA5; (B) The expression of relevant adaptor protein molecules contained the MyD88, TRIF and IPS-1; (C) The expression of proinflammatory cytokines (IL-1β, IL-2, IL-6 and IL-8); (D) The expression of type I and II IFNs and ISGs (Mx, PKR and OAS). Differences were analyzed with Student’s t test and were considered significant as follows: *P < 0.05; **P < 0.01.
