Figure 3
From: The long noncoding RNA ASNR regulates degradation of Bcl-2 mRNA through its interaction with AUF1
Lnc_ASNR interacts with AUF1.
(A) Pull-down assay enriches Lnc_ASNR RNA 20-fold. All relative abundances were compared to 10% input. (B) Western blot of AUF1 pull down. The AUF1 antibody exhibits the four isoforms (p45, p42, p40, p37). (C) Native RNA immunoprecipitation (RIP) of AUF1 enriches Lnc_ASNR. U1 and GAPDH are negative controls. All relative abundances were compared to 10% input. (D) Formaldehyde cross-linked RNA immunoprecipitation (RIP) of AUF1 enriches Lnc_ASNR. U1 and GAPDH are negative controls. All relative abundances were compared to 10% input. (E) Western blot of AUF1 in the RIP assay.
