Figure 4
From: The long noncoding RNA ASNR regulates degradation of Bcl-2 mRNA through its interaction with AUF1
Lnc_ASNR positively regulates Bcl-2.
(A) The Venn diagram indicates the 111 genes are expressionally changed in the two siRNAs mediated knockdown Lnc_ASNR (si-A represents si-Lnc_ASNR-1 and si-B represents si-Lnc_ASNR-2). The bottom panel show that the expression levels of overlapped 111genes (80 up-regulated and 31 down-regulated) were significantly altered in RKO cells when two independently siRNAs mediated knockdown of Lnc_ASNR (high expression: red, low expression: green). (B) The two representative gene sets (DEBIASI_APOPTOSIS_BY_REVIROUS_INFECTION_UP,REACTOME_REGUATION_OF_APOPTOSIS) of GSEA analysis are shown. (C) Formaldehyde cross-linked RNA immunoprecipitation (RIP) of AUF1 enriches Bcl-2. U1 and GAPDH are negative controls. All relative abundances were compared to 10% input. (D) Relative expression of AUF1, Bcl-2 and Lnc_ASNR after knock down of AUF1 by two different siRNAs (the two AUF1 siRNAs: siAUF1-1, siAUF1-2). (E) Western blot of AUF1 and Bcl-2 from D. (F) Relative expression of AUF1, Bcl-2 after knock down of Lnc_ASNR by two different siRNAs (F left). Western blot result of Bcl-2 in (F right). (G) Relative expression of Bcl-2 after overexpression of Lnc_ASNR (G left). Western blot result of Bcl-2 (G right).
