Figure 5
From: The long noncoding RNA ASNR regulates degradation of Bcl-2 mRNA through its interaction with AUF1
Lnc_ASNR suppresses Bcl-2 mRNA degradation through rising nucleus-retention of AUF1.
(A) Relative abundance of Bcl-2 mRNA (qRT-PCR) cells treated with α-amanitin 48 h after Lnc_ASNR knockdown by siRNA transfection in RKO cells. Error bars represent standard deviation (±SD) in triplicate experiments. (B) Relative abundance of Bcl-2 mRNA (qRT-PCR) with α-amanitin treatment 24 h after transfection with the Lnc_ASNR expression vector pcDNA3.1 (+) in HCT116 cells. Error bars represent standard deviation (±SD) in triplicate experiments. (C) Western blots showing AUF1 protein expression in whole-cell lysates after knockdown (RKO cells) or overexpression (HCT116 cells) of Lnc_ASNR. (D) Western blots showing the distribution pattern of AUF1 protein in RKO cells after siRNA transfection. GAPDH and Lamin B1 were used as loading controls for proteins isolated from cytoplasmic and nuclear fractions, respectively. (E) Western blots showing the distribution pattern of AUF1 protein in RKO cells after pcDNA3.1 (+) Lnc_ASNR transfection. For GAPDH and Lamin B1 see D.
