Table 1 Primer sequences used for real-time-PCR analysis.
From: Melatonin prevents acute kidney injury in severely burned rats via the activation of SIRT1
mRNA | Forward Primer | Reverse Primer: |
|---|---|---|
SIRT1 | 5′-TCGTGGAGACATTTTTAATCAGG-3′ | 5′-GCTTCATGATGGCAAGTGG-3′ |
TNF-α | 5′-TGCTTGTTCCTCAGCCTCTT-3′ | 5′-CAGAGGGCTGATTAGAGAGAGGT -3′ |
IL-1β | 5′-GACCTGTTCTTTGAGGCTGAC-3′ | 5′-TCCATCTTCTTCTTTGGGTATTGTT-3′ |
IL-10 | 5′-CAGAGAAGCATGGCCCAGAA-3’ | 5′-GCTCCACTGCCTTGCTCTTA -3′ |
ICAM-1 | 5′-CACAAGGGCTGTCACTGTTCA-3′ | 5′-CCCTAGTCGGAAGATCGAAAGTC-3′ |
BAX | 5′-GTGAGCGGCTGCTTGTCT-3′ | 5′-GGTCCCGAAGTAGGAGAGGA-3′ |
Bcl2 | 5′-GCGGA AGTCA CCGAA ATG-3′ | 5′-AGGAC AGCGA TGGGA AAA-3′ |
GAPDH | 5′-GGCACAGTCAAGGCTGAGAATG-3′ | 5′-ATGGTGGTGAAGACGCCAGTA-3′ |
