Figure 3: Statistical analysis of 1H-15N HSQC spectra of biosimilar filgrastim (blue) and originator filgrastim US (orange).
From: Biosimilar structural comparability assessment by NMR: from small proteins to monoclonal antibodies
(a) Overlay of the cross-peak positions in the pH 4.0 formulation for the t-test analogue evaluation. (b) Overlay of the biosimilar filgrastim at three different pH values: 3.0 (yellow), 4.0 (blue) and 4.4 (red). (c) Number of significantly shifted peaks of pairwise compared spectra in the pH 4.0, 4.4 and 3.0 formulations (0 peaks for pH 4.0 vs pH 4.0, 1 peak for pH 4.0 vs pH 4.4, 3 peaks for pH 3.0 vs pH 4.0 and 7 peaks for pH 3.0 vs pH 4.4 formulation).
