Figure 5: Histograms of peak-shift distances between the biosimilar and originator filgrastim products.
From: Biosimilar structural comparability assessment by NMR: from small proteins to monoclonal antibodies
Tolerance intervals for the normal, Cauchy and Gamma distributions are shown as blue, green and orange dashed lines, respectively. (a) 1H chemical shift difference histogram of originator filgrastim US and EU batch in the pH 4.0 formulation. (b) 1H chemical shift difference histogram of biosimilar filgrastim batch 1 and originator US batch in the pH 4.0 formulation. (c) biosimilar filgrastim batch 2 in pH 4.0 and pH 3.0 formulation. (d) Gamma distribution of the weighted chemical shift differences of the originator filgrastim US and EU batch in the pH 4.0 formulation. (e) biosimilar filgrastim batch 1 and originator US batch in the pH 4.0 formulation and (f) biosimilar filgrastim batch 1 in pH 4.0 and pH 3.0 formulation.
