Figure 6: NOESY spectra of the full size monoclonal antibody rituximab (rtx. biosimilar and the reference product) and image difference analysis.
From: Biosimilar structural comparability assessment by NMR: from small proteins to monoclonal antibodies
(a) Processed NOESY spectrum of biosimilar rituximab batch 1, (b) of originator rituximab reference product sample and (c) difference spectrum with region 1 (aliphatic) and 2 (amide-aromatic) and region for the noise level determination. (d) Spectral difference scores for the comparison of the reference and biosimilar rituximab region 1. (e) Spectral difference scores for the comparison of the biosimilar rituximab at three different pH values.
