Figure 2

Sulforaphane attenuated rotenone-induced dopaminergic neurodegeneration.

(a) Immunohistochemistry with anti-tyrosine hydroxylase (TH) antibody in the brain. Pictures show 30 μM-thick sections of cerebral cortex and striatum between different groups. (b) Immunoblots of cerebral cortex protein extracts blotted with TH and GAPDH antibodies. (c) Immunoblots of striatal protein extracts blotted with TH and GAPDH antibodies. (d) Densitometric quantification of TH-positive neurons and fibres. (e) Blots for TH were semi-quantified using NIH ImageJ. Relative density expressed as the ratio TH/GAPDH. Each value is presented as mean ± SEM, n = 3. ^aP < 0.01 vs. control group; ^bP < 0.05, ^cP < 0.01 rotenone group vs. rotenone + sulforaphane treatment group.