Figure 4

Sulforaphane inhibited rotenone-induced neuronal apoptosis by restoring mTOR pathway activity.

Representative immunoblots for p-mTOR, mTOR, p-p70S6K, p-70S6K, 4E-BP1 and GAPDH in cerebral cortex (a) and striatum (b). Blots for p-mTOR, p-p70S6K and 4E-BP1 were semi-quantified using NIH imageJ. Relative density expressed as the ratios of p-mTOR/GAPDH (c), p-p70S6K/GAPDH (d) and 4E-BP1/GAPDH (e) in cerebral cortex and striatum, respectively. Representative immunoblots for cleaved-caspase-3 in cerebral cortex (f) and striatum (g). Blots for cleaved-caspase-3 were semi-quantified using NIH imageJ. Relative density expressed as the ratio cleaved-caspase-3/GAPDH (h). Each value is presented as mean ± SEM, n = 3. ^aP < 0.05, ^bP < 0.01 vs. control group; ^cP < 0.05, ^dP < 0.01 rotenone group vs. rotenone + sulforaphane treatment group.