Figure 4 | Scientific Reports

Figure 4

From: Improving the Production of L-Phenylalanine by Identifying Key Enzymes Through Multi-Enzyme Reaction System in Vitro

Figure 4

Determination of enzyme activity of AroK and AroL in vitro.

(a) The enzyme activity of AroK; (b) The enzyme activity of AroL. The activities of the purified shikimate kinase was determined by coupling the release of ADP from the shikimate kinase-catalyzed reaction to the oxidation of NADH using pyruvate kinase and lactate dehydrogenase as coupling enzymes. Shikimate-dependent oxidation of NADH was monitored at A340 (ε = 6,200 M−1cm−1) at 25 °C. The data are from three experimental replicates.

Back to article page