Figure 6
Determination of the absolute enzyme concentrations.
(a) Relative ratios of the enzymes in the shikimate acid pathway. Original proteomics data was processed by APEX method. The relative amounts of enzymes in shikimate acid pathway were normalized to AroK. The data are from three experimental replicates. (b) The determination of AroK absolute concentration in the crude enzyme extract. Different amounts of purified AroK were added into the diluted crude extract and the reaction rates were determined. The AroK concentration in the diluted crude extract was calculated through the intersection with X-axis and the absolute concentration of AroK in the crude enzyme extract was calculated through the dilution rate. The data are from three experimental replicates.
