Table 2 The determination of purified enzyme activity.

From: Improving the Production of L-Phenylalanine by Identifying Key Enzymes Through Multi-Enzyme Reaction System in Vitro

 

Enzyme fraction

Phenylpyruvate formed (μM)

Control

aroL, aroA

0

Part 1

aroL, aroA, aroC, pheA

0.26

Part 2

tyrB

0.32

  1. Incubation mixture was described in the “Assay of the enzyme activities”. Phenylpyruvate was determined as described in the “Determination of phenylpuruvate acid”.
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