Figure 1: Myeloid-specific RBP-J deletion alleviated hepatic I/R injury in vivo. | Scientific Reports

Figure 1: Myeloid-specific RBP-J deletion alleviated hepatic I/R injury in vivo.

From: Blocking Notch signal in myeloid cells alleviates hepatic ischemia reperfusion injury by repressing the activation of NF-κB through CYLD

Figure 1

(a,b) Liver morphology and function in RBP-J cKO mice. Lyz2-Cre transgenic mice were crossed with RBP-Jf/+ mice to obtain Lyz2-Cre-RBP-J+/+ (Ctrl) and Lyz2-Cre-RBP-Jf/f (cKO) mice. Liver sections were made from 6-weeks-old mice and stained by H&E staining (a). Serum ALT (left) and AST (right) were examined and compared between the cKO and control groups (b). (c,d) RBP-J cKO and control mice were subjected to hepatic ischemia, and examined 6 h post reperfusion. Liver sections were stained by H&E staining, with the outlined areas showing hepatic necrosis (c upper) and the necrosis area was quantified (c lower). Serum ALT (left) and AST (right) were determined (d). Bars = mean ± SD (n = 5). *P < 0.05, **P < 0.01, ns, not significant.

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