Figure 2: RBP-J disruption in myeloid cells led to decreased apoptosis after hepatic I/R injury. | Scientific Reports

Figure 2: RBP-J disruption in myeloid cells led to decreased apoptosis after hepatic I/R injury.

From: Blocking Notch signal in myeloid cells alleviates hepatic ischemia reperfusion injury by repressing the activation of NF-κB through CYLD

Figure 2

RBP-J cKO and control mice were subjected to hepatic ischemia, and analyzed 6 h post reperfusion. (a) Liver sections were stained with TUNEL (left), and the number of apoptotic cells was determined and compared quantitatively between the RBP-J cKO and control mice (right). (b) Total protein of liver was prepared. The protein level of cleaved caspase-3 p17 and caspase-3 were evaluated by using Western blot, with β-actin as a reference control (left). The bands were quantitatively compared between the RBP-J cKO and control mice (right). Bars = mean ± SD (n = 5). *P < 0.05, **P < 0.01.

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