Figure 3: Myeloid-specific RBP-J deletion induced attenuated inflammation in liver after I/R injury. | Scientific Reports

Figure 3: Myeloid-specific RBP-J deletion induced attenuated inflammation in liver after I/R injury.

From: Blocking Notch signal in myeloid cells alleviates hepatic ischemia reperfusion injury by repressing the activation of NF-κB through CYLD

Figure 3

RBP-J cKO or control mice were subjected to hepatic ischemia, and analyzed 6 h post reperfusion. (a) Liver sections were immunostained with the anti-MPO antibody (left), and MPO-positive cells were quantitative compared between the RBP-J cKO and control mice (right). (b) Total RNA was extracted from liver tissue. The mRNA level of TNF-α (left) and IL-1β (right) was analyzed by using real-time RT-PCR, with β-actin as a reference control. (c) Serum TNF-α (left) and IL-1β (right) level was determined by using ELISA. Bars = mean ± SD (n = 5). *P < 0.05, **P < 0.01.

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