Figure 4
Antimicrobial activity of Aβx-42.
Flow cytometry analysis of E. faecalis, E. coli, S. aureus, L. monocytogenes and C. albicans after 6 h of incubation with the indicated Aβ peptide variants at a concentration of 25 μg/ml (gray) or 50 μg/ml (black). Column one (control) depicts representative density plots of forward scatter vs. autofluorescence at 525 nm in untreated microorganisms gated for DAPI positivity. Column two (Aβ3p-42) shows the same cultures after incubation with Aβ3p-42. Column three (% AF+/FSC−) shows the percentage of microorganisms with increased autofluorescence at 525 nm or reduced forward scatter (upper left quadrant) which are supposed to be damaged. The results are depicted as scatter plots with the median ± interquartile range. Cultures treated with 25 μg/ml and 50 μg/ml Aβ-peptides were grouped for the statistical analysis. Asterisks indicate significant differences calculated by the Friedman test followed by Dunn’s post-test. *p < 0.05, **p < 0.01, ***p < 0.001.
