Figure 2: Chronic depletion of BI-1 increases hepatic CYP2E1 activity and promotes hepatic ER stress and ROS accumulation in high-fat diet-fed mice. | Scientific Reports

Figure 2: Chronic depletion of BI-1 increases hepatic CYP2E1 activity and promotes hepatic ER stress and ROS accumulation in high-fat diet-fed mice.

From: Effect of BI-1 on insulin resistance through regulation of CYP2E1

Figure 2

(a) Western blot analysis showing effects of BI-1 depletion on expression levels of CYP2E1 and CPR in livers of BI-1 wild-type (BI-1+/+) (n = 5) and BI-1 knock-out (BI-1−/−) (n = 5) mice. (b) Co-immunoprecipitation assay of interaction between CPR and CYP2E1 in livers of BI-1 wild-type and BI-1 knock-out mice. (c) Estimation of chlorozoxane hydroxylase activity of CYP2E1 and (d) lipid peroxidation showing ER-stress induced ROS production in livers of BI-1 wild-type (BI-1+/+) (n = 5) and BI-1 knock-out (BI-1−/−) (n = 5) mice. (e) Western blot analysis showing effects of chronic depletion of BI-1 on ER stress signaling pathways in livers of high-fat diet-fed mice. Data in (c–e) represent mean ± SD (*p < 0.05, **p < 0.005, ***p < 0.0005, t-test).

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