Figure 8: CYP2E1 promotes palmitate-induced ER stress and insulin resistance.
From: Effect of BI-1 on insulin resistance through regulation of CYP2E1
(a) DCF-DA assay showing palmitate-induced ROS production in Neo and CYP2E1 cells. (b) Western blot analysis demonstrating effects of CYP2E1 expression on palmitate-induced ER stress response. (c,d) Neo and CYP2E1 cells were treated with 250 μM palmitate in presence or absence of 100 nM insulin. Western blot analysis showing effects of CYP2E1 on tyrosine and serine 307 phosphorylation of IRS1 and IRS2, and quantification of tyrosine and serine 307 phosphorylation of IRS2 (c) and its downstream proteins (d). Data in (a,c) (n = 4) represent mean ± SD (*p < 0.05, **p < 0.005, ***p < 0.0005, t-test). (b,d), Representative data performed in three separate measurements.
