Figure 4: miR-195-5p reduces DLL1 expression by directly targeting its 3′UTR. | Scientific Reports

Figure 4: miR-195-5p reduces DLL1 expression by directly targeting its 3′UTR.

From: Identification of microRNA-regulated pathways using an integration of microRNA-mRNA microarray and bioinformatics analysis in CD34+ cells of myelodysplastic syndromes

Figure 4

(A) miR-195-5p was up-regulated in patients with MDS-RCMD and down-regulated in the K562 and SKM-1 cell lines. (B) DLL1 was down-regulated in patients with MDS-RCMD and up-regulated in the K562 and SKM-1 cell lines. (C,D) Overexpression of miR-195-5p significantly decreases the expression of DLL1 mRNA and protein in SKM-1 and K562 cells. (E) The representative flow graphic for DLL1 detection is shown. (F) A series of 3′UTR fragments of DLL1, including full length, binding site 1, binding site 2, and their corresponding mutant counterparts, were directly fused downstream of the firefly luciferase gene. (G) miR-195-5p post-transcriptionally reduces DLL1 expression by directly targeting its 3′UTR. Error bars throughout represent the s.e.m. *P < 0.05; **P < 0.01 and ***P < 0.001. Unpaired Student’s t tests were used to calculate the P value in comparison analyses. All experiments were replicated three times.

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