Table 1 Characteristic peaks of IR standard spectra for three different HPLC solvents and NaH2PO4.

From: Separation and determination of estrogen in the water environment by high performance liquid chromatography-fourier transform infrared spectroscopy

Mobile phase

Characteristic Peaks (cm−1)

methanol

663.62

1029.39

1115.32

1449.9

2044.68

-CHO)

(δas -CH3)

2522.34

2832.48

2944.75

3341.19

 

-CH3)

-CH3)

-OH)

 

acetonitrile

749.31

918.88

1039.25

1376.03

1446.51

(δs-CH3)

(δas -CH3)

2252.43

2292.35

2944.08

3002.76

3164.29

-CN)

-CH3)

H2O

699.76

1646.77

2140.01

3424.1

 

H2O)

H2O)

H2O)

 

NaH2PO4

874.43

934.39

991.36

1060.21

1130.45

P-OH)

P-OH)

(νas-PO4)

(νs-PO2)

1165.18

1287.51

2399.59

2782.90

 

(νas-PO2)

-PH)

(νP-OH)

 
  1. ν: stretching vibration δas: asymmetrical deformation vibration γ: out-plane flexural vibration.
  2. δs: symmetrical deformation vibration ρ: rocking vibration δ: deformation vibration.
  3. νas: asymmetric stretching vibration νs: symmetrical stretching vibration.