Figure 2 | Scientific Reports

Figure 2

From: Multiplex gene editing via CRISPR/Cas9 exhibits desirable muscle hypertrophy without detectable off-target effects in sheep

Figure 2

Detection of the sgRNA:Cas9-mediated off-target cleavage sites in sheep fibroblasts.

The PCR products of the potential off-target sites in MSTN, ASIP and BCO2 sgRNA:Cas9 loci from sheep fibroblasts (a,b). 17 predicted off-target sites that were most homologous to MSTN, ASIP and BCO2 sgRNAs were named OT1 to OT17. (c,d) Detection of the sgRNA:Cas9- mediated off-target cleavage of MSTN, ASIP and BCO2 by using the T7EI cleavage assay. All PCR products from (a,b) were subjected to T7EI assay.

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