Figure 4
Phenotypic analyses of gene-modified sheep targeting MSTN.
(a) Birth weight of the MSTN-disrupted and WT lambs. Red dots indicate MSTN-disrupted male founders; red triangles indicate MSTN-disrupted female founders. Blue dots indicate male founders with more than two genes (including MSTN) disrupted, blue triangles indicate female founders with more than two genes (including MSTN) disrupted. Black dots indicate male WT, blue triangles indicate female WT. (b) Body weight changes in MSTN-disrupted and WT sheep from D0 to D240. *p < 0.05, **p < 0.01, student’s t-test. (c) The average daily gain (ADG) of MSTN-disrupted and WT sheep from D0 to D240. (d) H&E staining shows the morphology of myofibers in the muscles of WT and MSTN-disrupted (MUT) (#29) sheep. (e) TEM analyses of muscle tissues of a mutant (#29) and WT sheep at D120. Scale bars: left = 2 μm. (f) The diameter of myofibers for a mutant (#29) and WT sheep. (g) Western blotting analysis using anti-MSTN and anti-GAPDH (loading control) antibodies in a MSTN-disrupted sheep (MUT(#29)) and WT (#504).
