Table 1 The efficacy of Cas9-mediated modifications in sheep fibroblasts, embryos and founder animals.

From: Multiplex gene editing via CRISPR/Cas9 exhibits desirable muscle hypertrophy without detectable off-target effects in sheep

sgRNA

Fibroblasts

Injected embryos

Tested live lambs

MSTN-sg1

8/13 (61.5%)

7/20 (35.0%)

10/36 (27.8%)

MSTN-sg2

12/15 (80.0%)

ASIP-sg1 and 2

6/12 (50.0%)

10/20 (50.0%)

12/36 (33.3%)

BCO2- sg1 and 2

5/7 (71.4%)

10/20 (50.0%)

10/36 (27.8%)

MSTN&ASIP&BCO2

—

4/20 (20%)

2/36 (5.6%)

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