Figure 2: Effect of recombinant proteins on cell survival in PC 12 cells by CCK-8 assay.

(a) More living cells were found in the neurotrophic factors with NtA, when the same quality recombinant proteins were provided. (b) More living cells were found in only LBD-GDNF added than only LBD-BDNF (P < 0.05). Whatever ratios of LBD-BDNF and LBD-GDNF mixed are preferable than single usage of LBD-BDNF or LBD-GDNF (P < 0.05), and the most living cells were found when the ratio of LBD-BDNF to LBD-GDNF was 2:3 (P < 0.05). The ratio of 0 or 10 indicates cells were cultured in the mentioned conditions without LBD-BDNF or LBD-GDNF. n = 6. *P < 0.05.