Figure 5

YL1 Expression Pattern Analysis.
(a) qRT-PCR analysis of YL1 expression in various tissues, including roots (R), stem (S), leaves (L), sheath(Sh), spike for mature stage (MS) and young spike (YS) were detected. The YL1 transcript level was normalized to the Actin gene transcript (LOC_Os03g50885). (b) pYL1::GUS expression patterns in transgenic rice plants. From left to right: young buds, root, leaves, stems, leaf sheath and young spike. (c) qRT-PCR analysis of the YL1 expression in different-aged leaves. Leaf1 to Leaf5 represent leaves from the youngest to the oldest ones in 80-day-old wild-type plants grown under field condition (see Supplemental Fig. S6). (d) qRT-PCR analysis of the YL1 expression during greening of etiolated seedlings. Wild type rice seeds were germinated and grown in darkness for 7 d, etiolated seedlings were then illuminated for 0 (control), 1, 3, 6, 12 and 24 h under normal light condition. All the data are means ± SD (n = 3).