Figure 2: STIM1 and Orai1 mediate Ca2+ entry evoked by cockroach allergen extracts.
From: Allergens stimulate store-operated calcium entry and cytokine production in airway epithelial cells
(A) Western blot showing STIM1 and Orai1 expression in BEAS-2B cells and the effects of siRNA knockdown of STIM1 or Orai1. (B) Ca2+ traces showing the effects of siRNA knockdown of STIM1 and Orai1 on cockroach allergen-induced Ca2+ signals. A scrambled siRNA sequence was used as control. (C,D) Summary of average cytosolic Ca2+ levels 798 seconds after addition of cockroach allergen extract (8 μg/mL) (C) and the integral [Ca2+] signal following addition of cockroach extract (D). (E–G) Ca2+ imaging traces of individual cells treated with either a scrambled control siRNA (E) or siRNA against Stim1 (F) or Orai1 (G) showing effects of the knockdown on cockroach allergen-induced Ca2+ signals. Data are mean ± SEM of 29–38 cells. Representative of 3 independent experiments. **P < 0.01.
