Figure 3: Dust mite allergen extracts activate CRAC channels in BEAS-2B cells.

(A) HDM extract (nDerP, 18 μg/mL) induces a cytosolic Ca²⁺ signal that is blocked by BTP2. (B,C) Summary of the average rise in [Ca²⁺_i] 200 seconds after addition of HDM extract (B) and the integral Ca²⁺ signal (D–F) Knockdown of STIM1 or Orai1 inhibits nDerP induced Ca²⁺ signals. (D) Average trace showing the effects of siRNA mediated knockdown of STIM1 and ORAI1 on HDM allergen-induced Ca²⁺ signals. Summary of (E) average cytosolic Ca²⁺ levels 540 seconds after addition of dust mite allergen extract and (F) integrated Ca²⁺ signal. Data are mean ± SEM of 32–57 cells. Representative of 3–5 independent experiments. **P < 0.01, ***P < 0.001, nDerP, dust mite extract.