Table 1 Effect of insect and fungal allergen extracts on SOCE in BEAS-2B cells.

Allergen	Concentration tested (μg/mL)	Known Receptor	References	SOCE activation?
Cockroach extract	5–10	PAR2	9, 10	++
Dust mite extract	12–18	PAR2, other protease receptors	8, 30, 45	++
Der p1	5–20	PAR2	8	−
Aspergillus fumigatus	3.2–10	PAR2	46	−
Alternaria alternata	20–30	PAR2, ATP	32, 23, 47	−
Chitinase from Streptomyces griseus	20–30	PAR2	22	+

BEAS-2B cells were exposed to the indicated allergen extracts in a Ca²⁺ free Ringer’s buffer followed by readdition of 2 mM Ca²⁺ Ringer’s solution to induce SOCE. The amplitude of cytosolic Ca²⁺ rise, [Ca²⁺]_i was examined following addition of 2 mM Ca²⁺ by Ca²⁺ imaging using Fura2 dye. Cells were considered responders if the [Ca²⁺]_i elevation was >2 × SEM above the resting [Ca²⁺]_i. (+, Avg. response <2x above baseline [Ca²⁺]_i, ++, Avg. response >2x above baseline [Ca²⁺]_i).

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