Table 3 Primers sequence used for Real-time PCR.

From: Stimulatory effects of the degradation products from Mg-Ca-Sr alloy on the osteogenesis through regulating ERK signaling pathway

Gene

Primer sequence

Forward

Reverse

RUNX2

5′-TCCAACCCACGAATGCACTA-3′

5′-GAAGGGTCCACTCTGGCTTTG-3′

OSX

5′-AGAAGCCATACGCTGACCTTTC-3′

5′-AGTGAGGGAAGGGTGGGTAGTC-3′

Integrinα5

5′-AGCAACTGCACCTCCAACTACA-3′

5′-ACACTTGGCTTCAGGGCATT-3′

Integrinβ1

5′-TTGGTCAGCAACGCATATCTG-3′

5′-CAGCAAAGTGAAACCCAGCAT-3′

ALP

5′-GTCATCATGTTCCTGGGAGA-3′

5′-GGCCCAGCGCAGGAT-3′

BSP

5′-AATGGCCTGTGCTTTCTCGAT-3′

5′-CCCTGGACTGGAAACCGTTT-3′

COL I

5′-GCAGGGTTCCAACGATGTTG-3′

5′-AGGAACGGCAGGCGAGAT-3′

OCN

5′-CAATAAGGTAGTGAACAGAC-3′

5′-CTTCAAGCCATACTGGTCT-3′

OPN

5′-TCCAAAGCCAGCCTGGAAC-3′

5′-TGACCTCAGAAGATGAACTC-3′

GAPDH

5′-CATGGCCTTCCGTGTTCCTA-3′

5′-CCTGCTTCACCACCTTCTTGAT-3′

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