Figure 4
From: The CD63-Syntenin-1 Complex Controls Post-Endocytic Trafficking of Oncogenic Human Papillomaviruses
Syntenin-1 is required for papillomavirus infection.
(a) Syntenin-1 knockdown correlates with reduced HPV16 infectivity. Upper panels show efficiency of syntenin-1 knockdown in HeLa, HaCaT and NHEK cells (*non-specific band). Bottom panels show correlating HPV16 infection assay in HeLa, HaCaT and NHEK cells after syntenin-1 or control siRNA treatment. Infectivity was measured as in Fig. 1b. (b) HPV16 infectivity can be restored after syntenin-1 reexpression. HeLa cells were treated with syntenin-1 siRNAs, transfected with control or syntenin-1-expression plasmid and then infected with HPV16 PsV. Infectivity of HPV16 PsV was analysed as above. Upper panels show the efficiency of syntenin-1 knockdown and reexpression. *P < 0.05 significant decrease compared to control, $P < 0.05 significant increase compared to cells transfected with syntenin 3′UTR siRNA and control plasmid. (c) Syntenin-1 knockdown correlates with reduced HPV18 and HPV31 infectivity. HeLa cells were treated as in (a) and infectivity of HPV18 or HPV31 PsV was analysed as above.
