Figure 7
From: The CD63-Syntenin-1 Complex Controls Post-Endocytic Trafficking of Oncogenic Human Papillomaviruses
Ultrastructural analyses of HPV16-filled endosomes.
(a–d) HeLa cells were treated with CD63 or control siRNA and infected with HPV16 PsV. (a) Representative electron micrographs illustrating the morphology of virus containing endosomes (black arrow: HPV16 PsV; white arrow: intraluminal vesicles). Scale bar represents 200 nm. (b) For quantification of the size (diameter) of virus containing endosomes, 20 micrographs of target and control siRNA-treated cells were measured. (c) Representative electron micrographs of virus containing endosomes (diameter > 400 nm) after mock or HPV16 PsV infection. Scale bar represents 200 nm. (d) Quantification of viruses per vesicle with diameter >400 nm was determined by analysing 20 micrographs of control or target siRNA-treated cells, respectively. *P < 0.05 compared to control.
