Figure 8
From: The CD63-Syntenin-1 Complex Controls Post-Endocytic Trafficking of Oncogenic Human Papillomaviruses
Trafficking of HPV16 to multivesicular endosomes requires CD63/syntenin-1 complex.
(a) Representative pictures of CD63-GFP (green), LBPA (red) and HPV16 L1 (blue) triple staining with LBPA mAb and L1 pAb K75 antibodies. (b,c) Colocalisation analysis of HPV16 L1 and LBPA. HeLa cells were transfected with control siRNA, CD63 siRNA or syntenin-1 siRNA and infected with HPV16 PsV and stained with LBPA mAb and L1 pAb K75 antibodies. (b) Shows representative pictures of L1 (red) and LBPA (green) colocalisation. Nuclei are shown in blue. (c) Colocalisation of LBPA and L1 as well as early endosome marker EEA1 and L1 was analysed as in Fig. 3e (P = 0,062 for CD63 siRNA and 0,069 for syntenin-1 siRNA). (d,e) Colocalisation analysis of viral DNA and the Golgi. HeLa cells were transfected with control, CD63 or syntenin-1 siRNA and infected with EdU-HPV16 PsV for 7 or 12 hours. (d) Shows representative pictures of viral DNA (vDNA, green) and Golgi (red) 12 hours post infection. (e) Colocalisation of viral DNA and Golgi was analysed as above and normalized to infected control siRNA treated cells. *P < 0.05 compared to control.
