Figure 1: Time-courses of 125I-PrPSc level in the gastro-intestinal tract after p.o. administration. | Scientific Reports

Figure 1: Time-courses of 125I-PrPSc level in the gastro-intestinal tract after p.o. administration.

From: Prions efficiently cross the intestinal barrier after oral administration: Study of the bioavailability, and cellular and tissue distribution in vivo

Figure 1

(A) Levels of 125I-PrPSc in the stomach, duodenum, and jejunum at different times after p.o. administration. (B) Levels of 125I-PrPSc in the ileum and colon, showing peak levels of 125I-PrPSc at 3 hr and 6 hr after p.o. administration, respectively. Experimentally naïve mice were fasted for 16–20 hr prior to the experiment. Mice received a p.o. administration of 125I-PrPSc (1 × 106 cpm), and the whole blood, plasma, brain, liver, spleen, kidney, stomach, duodenum, jejunum, and colon was dissected. The radioactivity recovered from each tissue was further normalized by TCA precipitation to confirm the intactness of administered 125I-PrPSc, and data expressed as %ID/g of tissue. Pearson’s correlation of tissue levels of 125I-PrPSc to the stomach levels over time were: duodenum (R2: 0.9684, ***P < 0.0001), jejunum (R2: 0.8940, **P = 0.0013), ileum (R2: 0.07014, P = 0.5660), and colon (R2:0.1916, P = 0.3259), respectively. Mean values with standard error terms are presented. (n = 4–7 per point). ID: Injected dose.

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