Figure 6
From: SOX9-regulated cell plasticity in colorectal metastasis is attenuated by rapamycin
High SOX9 levels sensitize colorectal cancer cells to rapamycin.
(A) Representative immunoblot of phospho-S6 Ribosomal protein (Ser235/Ser236) (p-S6), SOX9 and β-actin protein expression in SW480 and SW620 cells treated with the indicated concentrations of rapamycin for 24 hours (n = 2). (B) Number of primary tumorspheres in SW480 and SW620 cells treated with vehicle (−) or rapamycin 10 nM (Rap) (n = 4). (C) Number of secondary tumorspheres derived from SW480 and SW620 primary tumorspheres treated with vehicle (−) or rapamycin 10 nM (Rap) (n = 4). (D) Number of tumorspheres formed by control and SOX9 overexpressing SW480 cells in the presence or absence of rapamycin 10 nM (Rap) (n = 3). (E) Number of tumorspheres formed by pLKO and shSOX9 SW620 cells in the presence or absence of rapamycin 10 nM (Rap) (n = 3).
