Figure 3: Study design and clinical and biological measurements.
Sketch and chart indicate exposure profile and timing of blood sampling and optic nerve sheath diameter (ONSD) measurements. At right in the upper figure, the optic nerve ultrasonography scan with hypoechogenic ocular globe and a longitudinal section of optic nerve. Measurements of ONSD were done 3 mm behind the papilla using an electronic caliper and an axis perpendicular to the optic nerve. At the bottom of the figure the blood sample preparation and electron paramagnetic resonance (EPR) acquisition protocol. CMH Spin Probe (50 μl) was added in equal amount (1:1) to the collected blood. The solution was immediately put in a glass EPR tube. From the generated radical compound, in the time course of the reaction, ten EPR spectra were collected in about 6 min, one of which is shown. The signal amplitude (a.u.) is proportional to the number of paramagnetic spin formed at the acquisition time. The calculated rate production values was converted in absolute levels (μmol·min−1) by using CP● radical as external standard.
