Figure 1
From: Identification of mutations through dominant screening for obesity using C57BL/6 substrains
Obesity screening of randomly mutagenized mice.
(a) C57BL/6J (B6J) males were treated with ethylnitrosourea (ENU) via intraperitoneal injections and crossed with C57BL/6N (B6N) females to generate the offspring (F1 mice). The F1 mice were screened for obesity. Sperm cells from obese mice were used for the in vitro fertilization of wild-type B6N eggs to obtain the N2 mice. The N2 progeny were examined for heritable obesity. If the pedigree showed heritable obesity, N2 mice were further used for a linkage analysis. (b) The histogram shows the body weight of all F1 mice screened at 18 weeks old. n = 2940.
