Figure 3
From: Identification of mutations through dominant screening for obesity using C57BL/6 substrains
Luciferase assay of the SIM1 protein.
(a,b) HEK293 cells were transiently transfected with wild type SIM1 and/or SIM1(M136K) expression plasmids, ARNT2 expression plasmids, SIM1 luciferase reporter plasmids and Renilla luciferase plasmids to assess transfection efficiency compensation. Bars indicate firefly/Renilla luciferase activity values relative to the mean firefly/Renilla values of the wild type SIM1 (a), 150 ng; (b), 50 ng)- and ARNT2-transfected cells. ***p < 0.001. ###p < 0.001 indicates P values for comparisons between the SIM1 (M315K) protein group and any of the wild-type SIM1 protein groups. One-way ANOVA followed by Tukey’s post-hoc test. Data are shown as the mean + s.e.m.
