Figure 4
From: Identification of mutations through dominant screening for obesity using C57BL/6 substrains
Metabolic phenotypes of Sim1 mutant mice.
(a) Both male and female Sim1M136K/+ mice (male n = 13, female n = 11) showed higher body weights than Sim1+/+ mice (male n = 12, female n = 13) at 26 weeks old in the N2 generation (Mann-Whitney U test, p < 0.0001). (b) Increased body weight of N3 mice indicates early-onset obesity in Sim1M136K/+ mice. Sim1+/+ mice, n = 19; Sim1M136K/+ mice, n = 10. *p < 0.05, ***p < 0.001, one-way repeated-measures ANOVA followed by Tukey’s post-hoc test. (c) Daily food intake of Sim1M136K/+ mice (n = 6) was higher than that of Sim1+/+ mice (n = 6) (d) Epididymal fat weights of Sim1M136K/+ mice (n = 6) and Sim1+/+ mice (n = 6). (e) Serum leptin levels of Sim1M136K/+ mice (n = 8) were higher than those of Sim1+/+ mice (n = 8). (f) Blood glucose levels were similar between Sim1+/+ mice (n = 19) and Sim1M136K/+ mice (n = 10). (g) Sim1M136K/+ (n = 8) mice had higher serum insulin levels than Sim1+/+ mice (n = 8). (h) Sim1 mRNA levels of the medial hypothalamus of Sim1M136K/+ mice (n = 6) was lower than those of Sim1+/+ mice (n = 6). *p < 0.05, ***p < 0.001, two-tailed t-test. Data are shown as the mean + s.e.m.
