Figure 6
From: Identification of mutations through dominant screening for obesity using C57BL/6 substrains
Metabolic phenotypes of Mc4r mutant mice.
(a) Both male and female Mc4rY41X/+ mice (male n = 11, female n = 4) showed higher body weights than Mc4r+/+ mice (male n = 11, female n = 8) at 26 weeks old in the N2 generation. (b) Increased body weight of N3 mice indicates early-onset obesity in Mc4rY41X/+ mice (n = 16) relative to Mc4r+/+ (n = 16) mice. ***p < 0.001, one-way repeated-measures ANOVA followed by Tukey’s post-hoc test. (c) Daily food intake of Mc4rY41X/+ mice (n = 6) was higher than that of Mc4r+/+ mice (n = 6) (d) Epididymal fat weights of Mc4rY41X/+ mice (n = 6) and Mc4r+/+ mice (n = 6). (e) Serum leptin levels of Mc4rY41X/+ mice (n = 8) were higher than those of Mc4r+/+ mice (n = 8). (f) Blood glucose levels were similar between Mc4r+/+ mice (n = 16) and Mc4rY41X/+ mice (n = 16). (g) Mc4rY41X/+ mice (n = 8) had higher serum insulin levels than Mc4r+/+ mice (n = 8). (h) Mc4r mRNA levels of the medial hypothalamus of Mc4rY41X/+ mice (n = 8) was higher than those of Mc4r+/+ mice (n = 6). *p < 0.05, **p < 0.01, ***p < 0.001, two-tailed t-test. Data are shown as the mean + s.e.m.
