Table 1 Whole-exome sequencing identifies non-synonymous mutations within the mapped chromosomal regions.
From: Identification of mutations through dominant screening for obesity using C57BL/6 substrains
Mouse ID | Obese-10 A | Obese-10 B | Obese-13 A | Obese-13 B | ||||
|---|---|---|---|---|---|---|---|---|
Body weight (26 weeks) | 56.3 g | 54.5 g | 62.5 g | 60.6 g | ||||
Total reads | 85,845,404 | 100.0% | 74,370,332 | 100.0% | 78,108,258 | 100.0% | 84,534,362 | 100.0% |
Mapped reads | 76,972,358 | 89.7% | 66,530,500 | 89.5% | 68,763,865 | 88.0% | 73,595,915 | 87.1% |
Not mapped reads | 8,873,046 | 10.3% | 7,839,832 | 10.5% | 9,344,393 | 12.0% | 10,938,447 | 12.9% |
Average coverage of capture target regions (x) | 93 | Â | 80 | Â | 81 | Â | 86 | Â |
Minimum coverage of capture target regions | ||||||||
 5x |  | 97.2% |  | 96.1% |  | 96.3% |  | 96.8% |
 10 x |  | 93.4% |  | 90.7% |  | 91.0% |  | 92.3% |
 20 x |  | 81.3% |  | 75.2% |  | 75.8% |  | 78.7% |
 40 x |  | 54.3% |  | 46.4% |  | 47.0% |  | 50.3% |
 80 x |  | 25.6% |  | 21.6% |  | 22.0% |  | 23.4% |
 100 x |  | 19.9% |  | 17.1% |  | 17.4% |  | 18.3% |
Variants called | 8,693 | 100.0% | 7,393 | 100.0% | 7,453 | 100.0% | 7,818 | 100.0% |
After filtering against control | 626 | 7.2% | 457 | 6.2% | 443 | 5.9% | 535 | 6.8% |
With amino acid change | 40 | 0.5% | 31 | 0.4% | 21 | 0.3% | 29 | 0.4% |
 Sec63 (chr10: 42816394) | A/A | A/A | A/G | A/G | ||||
 Sim1 (chr10: 50908536) | T/T | T/T | T/A | T/A | ||||
 Mc4r(chr18:66859918) | A/T | A/T | A/A | A/A | ||||
