Figure 4

Transcriptomics of male and female medaka livers investigated by RNA-seq approach.

Principal component analysis (PCA) performed according to the transcript count for the 16,523 genes encoding medaka liver proteins (Supplementary Table S4) from the 3 male and 4 female pooled cDNA sequenced by Hiseq 1000 comprising at least 30 million reads per libraries (A). Volcano plot representation of the gene expression according to female/male fold change average and significance of the differences (|log₂ FC| > 2 corresponds to |FC| > 4 and log₁₀ Pvalue < 3 corresponds to Pvalue < 0.001) (B). Female and male over-expressed genes are determined with positive and negative significant FC (F/M) values and are shown in red and blue, respectively. Top-25 lists of the male- and female-over-expressed genes (C and D, respectively).