Figure 4: Mean relative transcript levels for hexamerin-like proteins after knockdown in combination with protease inhibitor feeding. | Scientific Reports

Figure 4: Mean relative transcript levels for hexamerin-like proteins after knockdown in combination with protease inhibitor feeding.

From: Transcriptional Analysis of The Adaptive Digestive System of The Migratory Locust in Response to Plant Defensive Protease Inhibitors

Figure 4

Relative transcript levels in midguts and brains were examined for 5th larval locusts that received dsRNA for gfp as a control and were subsequently fed a control diet (open circles), or a diet containing SBBI and SBTI (open squares), and were compared to locusts that received a combination of dsRNA for LmHex2, 3 and 6 (open triangles). Hexamerin expression data for RNAi–control and RNAi–PI data were combined since no difference in transcript levels could be detected between these two groups. Relative transcript levels are normalized against two reference genes, rp49 and rps13. Axes are scaled logarithmically, and each horizontal dotted line represents a 10 fold difference in expression. RQ = relative quantity. Means ± SEM are represented (n = 5 pools of >5 individuals). Statistical differences were determined by ANOVA. For both brain and midgut, transcript levels were significantly reduced by RNAi (p < 0.01).

Back to article page