Figure 4: Effects of treatment with decitabine, ionizing radiation (IR), or their combination on IFN-γ production in T cells against target tumor cells.

T cells were co-cultured with decitabine, IR or both treated tumor cells (A549, HCT-116, and HepG2 cells) for 4 h (a). For blockade experiments (b), T cells were co-cultured with combination-treated target tumor cells in the presence of 10 ug/ml anti-CD40, CD80, and/or HLA-A,B,C antibody. Then the cell supernatants were harvested and analyzed by ELISA. Results express the average IFN-γ production ± SD in T cells co-cultured with A549, HCT-116, and HepG2 cells. Experiments were independently performed from five healthy donors. The assay was performed in triplicated each donor. Statistical significance was determined using a one-way ANOVA. ^#P < 0.05, ^##P < 0.005, ^###P < 0.0005 (^#DAC 0 versus other groups, mIgG versus other groups). *P < 0.05 (*DAC 5 versus other groups). ^**P < 0.05 (^**IR 8 Gy versus DAC 5 + IR 8 Gy). ^@P < 0.05, ^@@P < 0.005 (^@αCD40 + αCD80 versus αCD40 + αCD80 + αHLA-ABC).