Figure 7: Effects of treatment with decitabine, ionizing radiation (IR), or their combination on IFN-γ production in NK cells against target tumor cells.

Tumor cells treated with decitabine and/or IR were co-cultured with NK cells for 4 h. Then the cell supernatants were harvested and analyzed by ELISA. Results express the average IFN-γ production ± SD in NK cells co-cultured with A549, HCT-116, and HepG2 cells. Experiments were independently performed from five healthy donors. The assay was performed in triplicated each donor. Statistical significance was determined using a one-way ANOVA.