Figure 3 | Scientific Reports

Figure 3

From: Brown-like adipose progenitors derived from human induced pluripotent stem cells: Identification of critical pathways governing their adipogenic capacity

Figure 3

Differentiation of hiPSC-BAPs in optimized conditions.

(a) hiPSCs-BAPs were induced to differentiate in the defined medium for 25 days. Then cells were fixed and stained with Oil Red O for lipid droplets. Bar scale: 50 μm. (b) hiPSC-BAPs were induced to differentiate in the defined medium and expression of UCP1 and PLIN1 at different time after adipogenic induction was analyzed by Western-blot. (c) Stimulation of insulin pathway : hiPSCs-BAPs were induced to differentiate in the defined adipogenic medium for 28 days as described in the Materials and Methods and then stimulated with 100 nM insulin for 5 or 15 min. Proteins were prepared and analyzed for the indicated markers. (d) Stimulation of UCP1 expression by forskolin. hiPSCs-BAPs were induced to differentiate in the defined adipogenic medium for 25 days, then stimulated with 10 μM forskolin for 4 h. Values are means ± SEM. n = 4. E: Lipolysis induced by forskolin: hiPSCs-BAPs were induced to differentiate for 28 days, then stimulated with 10 μM forskolin for 5 h. *means p < 0.05 and **means p < 0.01.

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