Figure 2

(a,b) Characterization of material exchange processes related to stand-sit states of the microchip. Top-view images of a standing post (a) and sitting post (b) under a bright field microscope. (c) At the stand state, ducts are not formed because the barcoded glass slide is not touching the PDMS horizontal. (d) At the sit state, ducts may be open (sit-open, SO) or closed (sit-closed, SC). (e) Convective mass transfer of Dylight 488 from channels to chambers induced by the quick transition from SC to SO, which pushes dye molecules into chambers. (g) Diffusion of Dylight 488 quantitated by fluorescence intensity with time while the chip is in the SO state. The plot shows two cases for diffusion: net entry of dye molecules into chambers when dye concentration is higher in channels and net exit of molecules into channels when dye concentration is higher in chambers. (h) Fluorescence intensity of SA-647 in microchambers diminishes over time while the chip is in the SO state and dye concentration within microchambers is higher than that of channels. All error bars denote standard deviation from the mean. All scale bars are 100 μm.