Figure 5

(a) Application of the stand-sit microchip to single-cell assays with brain tumor cell line. Fluorescence images of vehicle U87 cells expressing EGFRvIII and the cells after Erlotinib treatment for 2 weeks in neural sphere culture are shown in the upper panel and their corresponding single-cell protein detection results are shown in the dot plots below. 1 cell and 0 cell results are compared for each protein. P values: P < 0.05 (*), P < 0.01 (**) and P < 0.001 (***), with P < 0.05 considered significant. (b) Correlation map of 6 proteins calculated from single-cell data on vehicle cells and drug treated cells. The colormap shown on the right indicates the correlation coefficient. Experiments are repeated two times, but the data in this figure are from the same chip to lower experimental variations.