Figure 2 | Scientific Reports

Figure 2

From: Local and Circulating Endothelial Cells Undergo Endothelial to Mesenchymal Transition (EndMT) in Response to Musculoskeletal Injury

Figure 2

Trauma induces endothelial marker expression in non-endothelial cells.

(A) Flow cytometry confirms relative absence of Scleraxis-cre cells in marrow (0.015% +/−0.007%); (B) Site of tissue harvest from Scx-cre/ROSA26mTmG mice; (C) Immunostaining of vessel from Scx-cre/ROSA26mTmG mice with VeCadherin, CD31 and Tie2 confirms that endothelial cells are not marked by the Scleraxis-lineage; (D) Immunostaining of tendon from Scx-cre/ROSA26mTmG mice with VeCadherin, CD31 and Tie2 confirms that cells of the Scleraxis-lineage within the hindlimb soft tissue are not endothelial cells; (E) Experimental design to mark non-endothelial, mesenchymal cells using Scx-creERT2/tdTomato + mice; (F) tdTomato + cells (ΔSCX) from Scx-creERT2/tdTomato + mice express VeCadherin after injury; (G) tdTomato + cells (ΔSCX) from Scx-creERT2/tdTomato + mice express CD31 after injury; (H) tdTomato + cells (ΔSCX) from Scx-creERT2/tdTomato + mice express Tie2 after injury. Yellow scale bars = 200 um.

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