Figure 4 | Scientific Reports

Figure 4

From: Local and Circulating Endothelial Cells Undergo Endothelial to Mesenchymal Transition (EndMT) in Response to Musculoskeletal Injury

Figure 4

(A) Schematic showing parabiosis of VeCadherin-cre/tdTomato mouse with unlabeled wild type mouse and injury on unlabeled wild type mouse; (B) tdTomato + (ΔVeCadherin) cells in parabiosis model express Osterix (OSX); (C) tdTomato + (ΔVeCadherin) cells in parabiosis model express SOX9; (D) Schematic showing tail vein injection of cultured, Cell-Vue-labeled (CV+) endothelial cells after trauma; (E) CV + endothelial cells are present in the injured site and express Osterix (OSX) with merge image in lower right quadrant; (F) CV + endothelial cells are present within the cartilage anlagen and express PDGFRα with merge image in lower right quadrant; (G) CV + endothelial cells are present within the cartilage anlagen and express SOX9 with merge image in lower right quadrant; (H) CV + endothelial cells are present within the cartilage anlagen and express Aggrecan (ACAN) with merge image in lower right quadrant; (I) H&E showing histologic character of CV + cells undergoing EndMT with chondrocyte appearance and PDGFRα expression as in (F).

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